- 作者: 陳水田 ; 翁啟惠
- 作者服務機構: 台灣大學生化科學研究所
- 中文摘要: Optically active 3-benzylthio-2-methylpropionic acid and 3-benzoylthio-2-methylpropionic acid have been prepared via protease-catalyzed hydrolysis of their corresponding ester and thioester. Subtilisin catalyzed the hydrolysis of the thioester of methyl 3-benzoylthio-2-methylpropionate twice as fast as that of the methyl ester derivative. The stability of the enzyme in organic cosolvents was studied. Immobilization of subtilisin on the solid support XAD-8 improved the stability of the enzyme . A practical preparative resolution of the title compound is reported.
- 英文摘要: --
- 中文關鍵字: Capoten; 3-Mercapto-2-Methylpropionic Acid; Protease Resolution; Subtilisin; Chymotrypsin; Ester Hydrolysis; Thioester Hydrolysis
- 英文關鍵字: 3-氫硫基-2-甲基丙酸;朊分解;枯草菌溶素;胰凝乳朊;酯類水解;硫酯水解