- 作者: Yung Hyun Choi and Hyun Soo Park
- 作者服務機構: Department of Biochemistry, Dongeui University College of Oriental Medicine, Busan, Republic of Korea
- 中文摘要: --
- 英文摘要:
Background: Diallyl trisulfide (DATS) is one of the major constituents in garlic oil and has demonstrated
various pharmacological activities, including antimicrobial, antihyperlipidemic,
antithrombotic, and anticancer effects. However, the mechanisms of antiproliferative activity
in leukemia cells are not fully understood. In this study, the apoptotic effects of DATS were
investigated in human leukemia cells.
Results: Results of this study indicated that treatment with DATS resulted in significantly inhibited
leukemia cell growth in a concentration- and time-dependent manner by induction of
apoptosis. In U937 cells, DATS-induced apoptosis was correlated with down-regulation of
Bcl-2, XIAP, and cIAP-1 protein levels, cleavage of Bid proteins, activation of caspases, and
collapse of mitochondrial membrane potential. The data further demonstrated that DATS
increased intracellular reactive oxygen species (ROS) generation, which was attenuated by
pretreatment with antioxidant N-acetyl-L-cysteine (NAC), a scavenger of ROS. In addition,
administration of NAC resulted in significant inhibition of DATS-induced apoptosis by
inhibiting activation of caspases.
Conclusions: The present study reveals that the cytotoxicity caused by DATS is mediated by generation of
ROS and subsequent activation of the ROS-dependent caspase pathway in U937 leukemia
cells. - 中文關鍵字: --
- 英文關鍵字: U937, DATS, Apoptosis, ROS, Caspase