- 作者: 劉明山; 劉瑞芬; 陳義雄; 陳森輝
- 作者服務機構: 國立臺灣大學理學院生化研究所; 中央研究院生化研究所; 國立臺灣大學醫學院小兒科
- 中文摘要:
,我們以含有1.0M尿素之等電荷焦點電泳法分離人體紅血球葡萄糖六磷酸去氫?,發現此酵素至少有七種異構?。
它們的等電點分別是6.88,6.79,6.64,6.50,6.39,6.19.6.10。再由第二度非連續電泳動分析,發現每一種異構?皆由
兩種蛋白質分子所組成。其中之一為雙體,分子量在94,000~98,000之間,另一個是單體,但也有可能是雙體經部份
分解之後的產物,分子量在50,600~66,800之間。
e - 英文摘要: Isoelectric focusing performed in 6.0% polyacrylamide gel in the presence of 1.0 M urea separateswell the various molecular forms of glucose-6-phosphate dehydrogenase from human erythrocytes. At leastseven sharp bands appear in the gel pattern, which vary both in isoelectric points and in the relative in-tensity. Their isoelectric points are at pH 6.88 for band 1, pH 6.79 for band 2, pH 6.64 for band 3, pH6.50 for band 4, pH 6.39 for band 5, pH 6.19 for band 6, and pH 6.10 for band 7. A second-dimensionaldisc electrophoresis performed in a polyacrylamide gel slab resolves each band into two components, aslow and a fast component defined according to their mobilities in the disc gel. The slow componentconstitutes the major portion of each band. All the seven slow components appear to be dimer having amolecular weight between 98,000-94,000. They belong to "charge isomers" having identical molecularsize but containing different net charges. The molecular weight of the fast components is between 66,800-50,600. These fast components might be monomer or the digested products of slow components.
- 中文關鍵字: human erythrocytes; glucose-6-phosphate dehydrogenase; isoenzymes; isoelectric focusing
- 英文關鍵字: --