- 作者: Jin-Ming Hwang; Tsui-Hwa Tseng; Yu-Ying Tsai; Huei-Jane Lee; Feu-Pi Chou; Chau-Jong Wang ; Chia-Yih Chu
- 作者服務機構: a Institute of Medicine, Medical College, b School of Applied Chemistry, Health Care and Management College, and c Institute of Biochemistry and Biotechnology, Medical College, Chung Shan Medical University, Taichung 402, Taiwan
- 中文摘要: --
- 英文摘要: Baicalein (BAL), a main flavonoid constituent of Scutellaria radix, was studied for its inhibitory effects on tert-butyl hydroperoxide (t-BHP)-induced hepatotoxicity and oxidative damage in primary cultures of rat hepatocytes. In a preliminary study, baicalein revealed effective antioxidant properties in a test of its capacity to quench the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH). Further investigations showed that baicalein, at the concentrations of 1, 5, and 10 μM, decreased the leakage of lactate dehydrogenase (LDH) and alanine aminotransferase (ALT) and the formation of malondialdehyde (MDA) induced by 30 min of pretreatment with t-BHP (1.5 mM) in primary cultures of rat hepatocytes. Baicalein also attenuated t-BHP-induced mitochondrial depolarization as determined by a retention test of rhodamine 123 and DNA repair synthesis as evidenced by unscheduled DNA synthesis (UDS). In addition, baicalein decreased the 8-hydroxy-2'-deoxyguanosine (8-OH-dG) content which acts as a DNA damage marker. The sum of the results suggests that the protective effect of baicalein against the cytotoxicity and genotoxicity of hepatocytes induced by t-BHP is due to its ability to quench free radicals.
- 中文關鍵字: --
- 英文關鍵字: baicalein, cytotonicity, genotonicity, hepatocyte, tert-butyl hydroperoxide, unscheduled DNA synthesis