- 作者: Hui-Ping Hsu and Jeng-Shong Shih
- 中文摘要:
Department of Chemistry, National Taiwan Normal University, Taipei, Taiwan 116, R.O.C.
A polymer coated piezoelectric crystal detection system with a home-made computer interface for signal acquisition and data processing was prepared as a liquid chromatographic detector for various proteins. Various polymers, e.g., polyvinylaldehhyde (polyacrolein) (PVA), polyacrylamide/glutaldehyde (PAA/GA) and bio-gel A, were used as coating materials on quartz crystals for adsorption of various protein molecules, e.g., catalase (CA), hemoglobin (Hb), a-chymotrypsin (Ch), albumin (Ab). The frequency responses of the polyacrlein coated piezoelectric detector for various proteins were in the order: catalase > hemoglobin > a-chymotrypsin > albumin. In contrast, the order of the frequency responses of bio-gel A and polyacrylamide/ glutaldehyde coated piezoelectric crystals for these proteins were: hemoglobin > catalase > a-chymotrypsin 3 albumin and hemoglobin > albumin > catalase. The polyacrolein coated piezoelectric crystal protein detector exhibited a good linear frequency response with a high sensitivity of about 2.5 ’ 103 Hz/(mg/mL) for catalase. In addition, bio-gel A and polyacrylamide/glutaraldehyde coated crystals were sensitive to hemoglobin with sensitivities of about 4.5 ’ 103 Hz/(mg/mL) and 3.0 ’ 103 Hz/(mg/mL), respectively. Study of the interference of various organic molecules, e.g., alcohols, amines, ketones and carboxylic acids, in the detection of proteins with theses polymer coated crystals was also made. The polyacrolein coated crystal for proteins underwent less interference from various organic molecules than bio-gel A or polyacrylamide/glutaraldehyde coated crystals. Effects of coating load, concentration of proteins and flow rate of liquid chromatographic eluent were also investigated and discussed. - 英文摘要: --
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- 英文關鍵字: --