• 作者： Ju, Yi-Hsu; Chiu, Chih-Kang; Huang, Fang-Cheng
• 中文摘要： 本研究以矽藻土吸附脂解酵素Rhizopus arrhizus製備固定化酵素,對棕櫚油分餾物與硬脂酸基質行酯交換反應。藉變更反應條件比較產物組,以探討反應最適條件。酯交換反應在系統水活性等於0.432,酵素載量等於10.33U/20mg Celite時具有最大活性。以合成可可奶油替代物而言,POMF與硬脂酸之莫爾比為1:3為最適基質比例,而基質濃度越低酵素的活性越高。其貯存穩定性在4.degree.C的貯存溫度下之半生期大約為26天。
• 英文摘要： The interesterification of palm oil midfraction (POMF) and stearic acid with Rhizopus arrhizus lipase immobilized on Celite was carried out in n-hexane. The water activity of the system was controlled by equilibrating the immobilized enzyme and substrate solution, separately, with a saturated salt solution of known water activity for at least 24 hours. The effects of water activity and enzyme loading on the interesterification reaction between POMF and stearic acid were investigated. It was found that a combination of water activity 0.432, enzyme loading 10.33U/mg Celite yields optimal interesterification activity. The effects of molar ratio of stearic acid to POMF and substrate concentration on the interesterification reaction were also examined. The half-life of the storage stability at 4.degree.C was 26 days.
• 中文關鍵字： 棕櫚油; 可可奶油替代物; 固定化脂解酵素; 水活性; 貯存穩定度; 酯交換
• 英文關鍵字： Rhizopus Arrhizus; Cocoa Butter Substitute; Immobilized Lipase; Water Activity; Storage Stability; Interesterification