- 作者： 王博仁; 林美霞
- 作者服務機構： 中央研究院植物研究所
- 中文摘要：
?樹屬闊葉一級木，生長快速，材質輕軟，有美麗紋理。唯採種不易，至今無法以人工發
芽，亦無實用之無性繁殖方法。
五年生之?樹天然下種苗之新芽，在含有 60 ppm kinetin 之修改 Linsmaier and Skoog
（LS）培養基下長成多數芽體。此芽可單獨培養長大發根而成個體，可移出管外成活。此法成功
率較低。
未成熟種子之子葉及芽體經無菌播種可以發芽成苗，其一至二月齡之苗，可作為繁殖之材
料。其頂芽在含有 IAA 1 ppm 之修改 LS 培養基下長根成苗。節芽在含有 5 ppm BA 之同樣
培養基下能長出一或多芽，而在含有 IAA 1 ppm 之同樣培養基下長根。經採取莖芽之實生苗由
基部長出新芽，如此可連續探芽五至七次，而由節芽長成之新芽及發根後之頂芽亦可用同樣地採
芽繁殖，此法由一實生苗可繁殖許多具有同樣遺傳性質之苗體。
經修改之 LS 培養基為：加二倍之鐵化合物，另加 glutamine, arginine 各50 ppm，malt
extract 100 ppm，糖 0.5%，adenine 5 ppm。培養條件為 18～20°C，2000 lux 白色日光燈，
12小時日長。
此等試管苗在滅苗之(石至)石，25°C（白天）15°C（夜間），12小時日長，4000 lux 白色日光
燈，蓋上打孔塑膠布（每 100 打 0.5 cm 直徑之圓孔），並澆以稀釋三倍之 knop 氏培養
液，此法可得 95%之成活率。u - 英文摘要： Sasafras randaiense is a valuable forestdeciduous species in Taiwan. Its lumber is ingreat demand for manufacturing high qualityfurniture. Owing to difficulties of seed germina-tion and rooting of its cuttings, the efforts tore-establish new sasafras through conventionalpropagation methods are futile. The only al-ternative left is the cloning by the modern invitro culture technique. The shoots of five-year-old sasafras seedlingcan be stimulated to grow multiple shoots onmodified Linsmaier and Skoog's medium (MLS)containing 60 mg/ kinetin. Formation of rootsis enhanced by the transfer of those shoots tothe liquid medium containing 5 to 10 mg/indolebutyric acid, supported with filter paperbridge. A terminal two-cm section from one totwo month-old seedling develops into completeplant on MLS medium supplemented with lmg/indolacetic acid. The upper part of axillarybud develops into a new terminal after the topbud is removed. The cutting of the new ter-minal shoot is made repeatedly. Emergence oflower axillary bud is stimulated in vitro on themedium supplemented with 5 mg/ benzylade-nine. The elongated acillary shoots can beused as cuttings to produce complete plants orused for starting a new culture to form moreaxillary bud. It is 95% successful to transfer the rootedplants to the sterilized vermiculite in growthchamber which controls the temperature of 25°C(day) and 15°C (night, 12 hr.), and the lightintensity of 4000 lux with fluorescent lamp.
- 中文關鍵字： --
- 英文關鍵字： --
