- 作者: Ying-Fang Su, Chi-Yen Liang, Chih-Yang Huang, Chih-Yu Peng, Claire Chiyu Chen, Ming-Cheng Lin, Rong-Kai Lin, Wei-Wen Lin, Ming-Yung Chou, Pao-Hsin Liao, Jaw-Ji Yang
- 作者服務機構: Institute of Medicine, School of Dentistry,Taichung, Taiwan R.O.C
- 中文摘要: --
- 英文摘要:
Background:
The DEP domain is a globular domain containing approximately 90 amino acids, which was first discovered in 3 proteins: Drosophila disheveled, Caenorhabditis elegans EGL-10, and mammalian Pleckstrin; hence the term, DEP. DEPDC1B is categorized as a potential Rho GTPase-activating protein. The function of the DEP domain in signal transduction pathways is not fully understood. The DEPDC1B protein exhibits the characteristic features of a signaling protein, and contains 2 conserved domains (DEP and RhoGAP) that are involved in Rho GTPase signaling. Small GTPases, such as Rac, CDC42, and Rho, regulate a multitude
of cell events, including cell motility, growth, differentiation, cytoskeletal reorganization and cell cycle progression.
Results:
In this study, we found that it was a guanine nucleotide exchange factor and induced both cell migration in a cultured embryonic fibroblast cell line and cell invasion in cancer cell lines; moreover, it was observed to promote anchorage-independent growth in oral cancer cells. We also demonstrated that DEPDC1B plays a role in regulating Rac1 translocated onto cell membranes, suggesting that DEPDC1B exerts a biological function by regulating Rac1. We
examined oral cancer tissue; 6 out of 7 oral cancer tissue test samples overexpressed DEPDC1B proteins, compared with normal adjacent tissue.
Conclusions:
DEPDC1B was a guanine nucleotide exchange factor and induced both cell migration in a cultured embryonic fibroblast cell line and cell invasion in cancer cell lines; moreover, it was observed to promote anchorage-independent growth in oral cancer cells. We also demonstrated that DEPDC1B exerts a biological function by regulating Rac1. We found that oral cancer samples overexpressed DEPDC1B proteins, compared with normal adjacent tissue. Suggest that DEPDC1B plays a role in the development of oral cancer. We revealed that proliferation was linked to a novel DEPDC1B-Rac1-ERK1/2 signaling axis in oral cancer cell lines. - 中文關鍵字: --
- 英文關鍵字: Anchorage-independent growth, Oral cancer, Extracellular-signal-regulated kinases, Rac1, DEPDC1B