- 作者: Dar-Chih Kuo; Su-Ping Hsu; Chiang-Ting Chien
- 作者服務機構: Department of Cardiovascular Surgery, Kuang-Tien General Hospital, Taichung, R.O.C.
- 中文摘要: --
- 英文摘要:
Increased reactive oxygen species (ROS) and hyperlipidemia can promote arterial thrombus. We evaluated the potential of a partially hydrolyzed guar gum (PHGG) as
dietary fiber on lipid profiles and FeCl3-induced arterial thrombosis in the high fat-diet fed hamsters. Our in vitro results found that PHGG is efficient to scavenge O2
—•, H2O2, and
HOCl. High fat-diet increased plasma triglyceride, total cholesterol, LDL, VLDL,
methylguanidine and dityrosine level and accelerated FeCl3-induced arterial thrombosis
formation (from 463±51 to 303±45 sec). Low dose PHGG supplement significantly
decreased the total cholesterol, LDL, methylguanidine and dityrosine level and delayed the
time for arterial thrombosis formation (528±75 sec). High dose PHGG supplement
decreased the level in triglyceride, total cholesterol, LDL and VLDL and further delayed
the time for arterial thrombus (671±36 sec). The increased Bax protein and decreased
Bcl-2 and HSP-70 protein expression was found in the carotid and femoral arteries of high
fat-diet hamsters. Low and high dose of PHGG supplement decreased Bax expression and
increased Bcl-2 and HSP-70 protein expression. We found that FeCl3 significantly
enhanced intercellular adhesion molecule-1 and 4-hydroxynonenal expression in the
endothelial site of damaged artery after 150-sec FeCl3 stimulation. PHGG supplement
decreased the endothelial ICAM-1 and 4-hydroxynonenal expression after 150-sec FeCl3
stimulation. Based on these results, we conclude that PHGG supplement can increase
antioxidant protein expression and thus decrease oxidative stress induced arterial injury. - 中文關鍵字: --
- 英文關鍵字: --