- 作者: 孫偉, 李小青, 焦奎
- 作者服務機構: College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology,Qingdao 266042, P. R. China
- 中文摘要: Room temperature ionic liquids (RTILs) N-butylpyridinium hexafluorophosphate (BPPF6) modified carbon paste electrode (CILE) was fabricated and applied to adsorb the hemoglobin (Hb) and TiO2 nanoparticles on the electrode surface step by step to form a Hb modified electrode noted as TiO2/Hb/ CILE. UV-Vis and FT-IR spectra showed that Hb in the film retained its native conformations. Cyclic voltammetric experiments indicated that a pair of well-defined quasi-reversible redox peaks appeared with the formal potential (E0¢) located at -0.251 V (vs. SCE) at pH 7.0 phosphate buffer solution (PBS), which was the characteristic of heme Fe(III)/Fe(II) redox couples. Electrochemical parameters of the Hb in the film such as the electron transfer coefficient (a), the electron transfer number (n) and the standard electron transfer rate constant (ks) were estimated as 0.469, 0.87 and 0.635 s-1, respectively.
- 英文摘要: --
- 中文關鍵字: Hemoglobin; TiO2 nanoparticles; Direct electrochemistry; Carbon ionic liquid electrode; N-butylpyridinium hexafluorophosphate (BPPF6).
- 英文關鍵字: --