- 作者: Henry J Tsai, Li-Ann Chi and Alice L Yu
- 作者服務機構: Genomic Research Center, Academia Sinica, Taipei, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
Background: Avian influenza H5N1 virus is highly pathogenic partially because its H5 hemagglutinin
contains a polybasic cleavage site that can be processed by proteases in multiple organs.
Methods: Monoclonal antibodies (mAb) specific to the synthetic peptide of hemagglutinin polybasic
cleavage site of H5N1 virus were raised and tested for their neutralizing potential.
Results: Purified mAb showed suppression of H5N1 pseudovirus infection on Madin-Darby Canine
Kidney (MDCK) cells but the efficacy was less than 50%. Since those mAb are specific to
the intact uncut polybasic cleavage site of hemagglutinin, their efficacy depends on the extent
of hemagglutinin cleavage on the viral surface.
Conclusions: Proteolytic analysis suggests the low efficacy associated with those mAb may be due to
proteolytic cleavage already present on the majority of hemagglutinin prior to the infection of
virus. - 中文關鍵字: --
- 英文關鍵字: Monoclonal antibody, Polybasic cleavage site, Hemagglutinin, H5N1 influenza