- 作者: 孫偉,焦奎,韓軍英,徐桂雲
- 作者服務機構: College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, P. R. China
- 中文摘要: In this paper the interaction of chromotrope 2B (Ch2B) with proteins was studied by the electrochemical method. Ch2B is an azo dye and shows irreversible electrochemical responses on the mercury electrode in a pH 3.0 Britton-Robinson (B-R) buffer solution. After the addition of human serum albumin (HSA) into the Ch2B solution, an interaction took place, and a supramolecular complex was formed in the mixed solution. The electrochemical parameters of the Ch2B-HSA interaction system were calculated and compared. The results showed that in the absence and presence of HSA in Ch2B solution, the electrochemical parameters such as the formal potential E0, the electrode reaction standard rate constant ks, etc. showed no significant changes, which indicated that an electro-inactive supramolecular biocomplex was formed. The free concentration of Ch2B in reaction solution was decreased, and this resulted in the decrease of the peak current. The binding constant and the binding ratio were calculated as 7.85 × 109 and 1:2, respectively, and the interaction mechanism was discussed. Based on the decrease of the peak current, this new electrochemical method was proposed for the determination of HSA in the concentration range of 2.0~25.0 mg/L with the linear regression equation as DIp" (nA) = 50.56C (mg/L) - 6.72 (g = 0.995). This method was further used to determine other different kinds of proteins, such as bovine serum albumin (BSA), oval albumin, etc.. The new method was successfully applied to detect the content of albumin in healthy human serum samples with the results in good agreement with the traditional Coomassie Brilliant Blue G-250 spectrophotometric method.
- 英文摘要: --
- 中文關鍵字: Chromotrope 2B; Serum albumin; Voltammetry; Interaction; Protein assay.
- 英文關鍵字: --