- 作者: Maud Wan-Ying Lee Alice Chien Chang Der-Shan Sun Chia-Yi Hsu Nan-Chi A. Chang
- 作者服務機構: Institutes of Microbiology and Immunology and Neuroscience, Center for Neuroscience, National Yang-Ming University, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要: A novel protein LUZP with 3 leucine zipper motifs at itsamino terminus is predominantly expressed in the adultbrain. A modified gene targeting approach was em-ployed in an attempt to establish in vitro and in vivomodels in which Luzp is knock-out (KO) for phenotypeassessment and a reporter gene lacZ is knock-in (KI) fortracing its expression. We report in this study the mo-lecular cloning of the Luzp gene, its targeting vectorconstruction and Luzp-KO/IacZ-KI embryonic stem (ES)clone selection. Since LUZP is also expressed in ES cells,the possibility of embryonic lethality cannot be excludedwhen attempting to establish Luzp-null mutant mice. Wehave therefore examined the development of homozy-gous Luzp-KOIIacZ-K1 clones in nude mice. Tissue typesderived from all three embryonic germ layers wereobserved in teratomas developed in nude mice. In situX-gal staining further revealed restricted expression ofLUZP in neural lineage cells.
- 中文關鍵字: --
- 英文關鍵字: Leucine zipper. Embryonic stem cell. Neural lineage