- 作者: Chi Zhang, Pingping Jia, Xupei Huang, Gian Franco Sferrazza, Gagani Athauda, Mohan P Achary, Jikui Wang, Sharon L Lemansi, Dipak K Dube, Larry F Lemanski
- 作者服務機構: Department of Biomedical Science, Florida Atlantic University, Boca Raton, FL, U.S.A.
- 中文摘要: --
- 英文摘要:
The Mexican axolotl, Ambystoma mexicanum, carries the naturally-occurring recessive
mutant gene 'c' that results in a failure of homozygous (c/c) embryos to form hearts that beat
because of an absence of organized myofibrils. Our previous studies have shown that a
noncoding RNA, Myofibril-Inducing RNA (MIR), is capable of promoting myofibrillogenesis
and heart beating in the mutant (c/c) axolotls. The present study demonstrates that the MIR gene
is essential for tropomyosin (TM) expression in axolotl hearts during development. Gene
expression studies show that mRNA expression of various tropomyosin isoforms in untreated
mutant hearts and in normal hearts knocked down with double-stranded MIR (dsMIR) are
similar to untreated normal. However, at the protein level, selected tropomyosin isoforms are
significantly reduced in mutant and dsMIR treated normal hearts. These results suggest that MIR
is involved in controlling the translation or post-translation of various TM isoforms and
subsequently of regulating cardiac contractility. - 中文關鍵字: --
- 英文關鍵字: --