- 作者: Chang-Tze R. Yu; Jih-Heng Li; Te-Chang Lee; Lih-Fang Lin
- 作者服務機構: 1 Graduate Institute of Biomedicine and Biomedical Technology, National Chi Nan University, Puli, Nantou. 545, Taiwan, R.O.C; ; 2 Department of Health, Executive Yuan, 100 Ai-Guo East Road, Taipei, 100, Taiwan, R.O.C, ; 3 Institute of Environmental Health Sciences, National Yang Ming University, Taipei, 112, Taiwan, R.O.C; ; 4 Institute of Biomedical Sciences, Academia S璯ica, Taipei, Taiwan, R.O.C; 5 Council of Agriculture, Executive Yuan, Taipei, 100, Taiwan, R.O.C
- 中文摘要: --
- 英文摘要: The sizes of organelles are tightly regulated in the cells. However, little is known on how cells maintain the homeostasis of these intracellular compartments. Using cocaine as a model compound, we have characterized the mechanism of deregulated vacuolation in cultured rat liver epithelial Clone 9 cells. The vacuoles were observed as early as 10 min following cocaine treatment. Removal of cocaine led to vacuole degeneration, indicating vacuolation is a reversible process. The vacuoles could devour intracellular materials and the vacuoles originated from late endosome/lysosome as indicated by immunofluorescence studies. Instant calcium influx and calmodulin were required for the initiation of vacuole formation. The unique properties of these late endosome/lysosome-derived vacuoles were further discussed. In summary, cocaine elicited a new type of deregulated vacuole and the involvement of calcium/calmodulin in vacuolation could shed light on prevention or treatment of cocaine-induced cytotoxicity.
- 中文關鍵字: --
- 英文關鍵字: cocaine, vacuolation, late endosome, calcium, calmodulin