- 作者: Phil-Sun Oh; Kye-Taek Lim
- 作者服務機構: #521, Molecular Biochemistry Laboratory, Institute of Biotechnology, Chonnam National University, 300 Yongbong-Dong, Kwangju, 500-757, South Korea
- 中文摘要: --
- 英文摘要: We investigated the preventive effect of glycoprotein (27 kDa) isolated from Gardenia jasminoides Ellis (GJE) fruits on colitis in dextran sulfate sodium (DSS, 3%)-induced A/J mice which were administrated orally for 7 days. Anti-inflammatory activity of GJE glycoprotein was assessed by neutrophil infiltration and colonic lipid peroxidation, and determined by myeloperoxidase (MPO) activity and levels of thio-barbituric acid reactive substances (TBARS), respectively in DSS treatment system. The activities of an-tioxidative enzymes [catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx)], activation of inflammation related mediators (iNOS, COX-2, and NF- κΒ), and production of nitric oxide (NO) and reactive oxygen species (ROS) were also measured. The results of this study showed that GJE glycoprotein (8μg/ml) has a scavenging property to inhibit the intracellular ROS production in RAW 264.7 cells and that GJE glycoprotein (80 mg/kg BW) significantly suppressed the increase in the MPO activity, TBARS level, and NO production, inflammation related mediators [iNOS, COX-2, and NF-κΒ (p50)] activity in DSS-induced mice. Interestingly, the activities of CAT, SOD, and GPx were gradually augmented after a supplement of GJE glycoprotein. Therefore, we suggest that GJE glycoprotein is preventive and therapeutic agent for the ulcerative colitis.
- 中文關鍵字: --
- 英文關鍵字: myeloperoxidase activity, reactive oxygen species, NF-κΒ (p50), GJE glycoprotein