- 作者: Ching-Yi Chu; Jerming Tseng
- 作者服務機構: Department of Biology, National Taiwan Normal University, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要: Induction of tumoricidal activity is one of the major func-tions of activated macrophages. Our previous studydemonstrated that P388D1 murine macrophage-like cellssecreted a plasmacytoma cytotoxic factor (PCF) that se-lectively killed certain tumor cell lines including MOPC-315 plasmacytoma in vitro. Our subsequent studiesdemonstrated that PCF killed MOPC-315 cells by induc-tion of apoptosis. In this report, the involvement of Fasand Fas ligand (FasL) in PCF-induced apoptosis wasinvestigated. Results suggest that expression of FasmRNA time-dependently increased in PCF-treated cellsand reached an optimal level after 36 h of treatment. Theaugmented effect of PCF on Fas mRNA expression wassignificantly reduced by the addition of CB7.C2, an anti-PCF monoclonal antibody. The expression of FasLmRNA was also induced by PCF and reached an optimallevel at 24 h, but sharply decreased after 36 h of treat-ment. Caspase-3 is one of the proteolytic enzymes thatcan be activated by the Fas-FasL interaction. In our stud-ies, the enzymatic activity of caspase-3 was significantlyinduced by PCF after 6 h of treatment and reached anoptimal level at 12 h. The augmented effect of PCF oncaspase activity was significantly reduced by the addi-tion of CB7.C2 and the caspase-3-specific inhibitor,DEVD-fmk. Therefore, PCF-treated plasmacytoma cellsmight undergo apoptosis via interaction between Fasand FasL.
- 中文關鍵字: --
- 英文關鍵字: --