- 作者: Yu-Chung chang Sung-Liang Yu wan-Jr Syu
- 作者服務機構: Institute of Microbiology and Immunology, and AIDS prevention and Research Center, National Yang Ming University, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要: The HIV pol sequentially encodes protease (PR), reversetranscriptase (RT), and integrase (IN) from the 5'-3' direc-tion. We explored the significance of this gene arrange-ment. All six possible gene dispositions were examined.In two situations where PR was removed from the lead-ing place and no two genes were in their original loca-tion, viral polyprotein processing was abolished. Pro-cessing of the polyprotein did not occur when IN wastranslocated to the front of PR-RT. However, in the fol-lowing two arrangements, the polyprotein was pro-cessed but only at specific sites. First, PR remained in theleading position while the locations of RT and IN wereexchanged: viral polyprotein was processed at a sitebetween the upstream transframe peptide (TF) and PR.Second, PR was placed after RT-IN and located at the dis-tal end of Pol. Processing occurred only at the createdjunction between TF and RT. These results indicated thatcleavage after TF occurred autocatalytically but did notproceed to a second site, which needed an extraneousPR for trans-action. Therefore, arranging Pol in the orderof PR-RT-IN warrants the streamline processing of thepolyprotein once the autocleavage is initiated.
- 中文關鍵字: --
- 英文關鍵字: Autoprocessing. Expression. HIV-1. Integrase. Protease. Reverse transcriptase