- 作者: Karen F Chambers; Joanna F Pearson; Davide Pellacani; Naveed Aziz; Miodrag Guzvic; Christoph A Klein; Shona H Lang
- 作者服務機構: Yorkshire Cancer Research Unit, Dept. Biology, University of York, Heslington, York, UK
- 中文摘要: --
- 英文摘要:
Background: Stromal signalling increases the lateral cell adhesions of prostate epithelial cells
grown in 3D culture. The aim of this study was to use microarray analysis to identify significant
epithelial signalling pathways and genes in this process.
Methods: Microarray analysis was used to identify genes that were differentially expressed when
epithelial cells were grown in 3D Matrigel culture with stromal co-culture compared to without
stroma. Two culture models were employed: primary epithelial cells (ten samples) and an epithelial
cell line (three experiments). A separate microarray analysis was performed on each model system
and then compared to identify tissue-relevant genes in a cell line model.
Results: TGF beta signalling was significantly ranked for both model systems and in both models
the TGF beta signalling gene SOX4 was significantly down regulated. Analysis of all differentially
expressed genes to identify genes that were common to both models found several morphology
related gene clusters; actin binding (DIAPH2, FHOD3, ABLIM1, TMOD4, MYH10), GTPase
activator activity (BCR, MYH10), cytoskeleton (MAP2, MYH10, TMOD4, FHOD3), protein binding
(ITGA6, CD44), proteinaceous extracellular matrix (NID2, CILP2), ion channel/ ion transporter
activity (CACNA1C, CACNB2, KCNH2, SLC8A1, SLC39A9) and genes associated with
developmental pathways (POFUT1, FZD2, HOXA5, IRX2, FGF11, SOX4, SMARCC1).
Conclusions: In 3D prostate cultures, stromal cells increase lateral epithelial cell adhesions. We
show that this morphological effect is associated with gene expression changes to TGF beta
signalling, cytoskeleton and anion activity. - 中文關鍵字: --
- 英文關鍵字: --