- 作者: Chao-Yi Wu; Archana Monie; Xiaowu Pang; Chien-Fu Hung; T-C Wu
- 作者服務機構: Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA
- 中文摘要: --
- 英文摘要:
Background: Effective vaccination against human papillomavirus (HPV) represents an opportunity to control
cervical cancer. Peptide-based vaccines targeting HPV E6 and/or E7 antigens while safe, will most likely require
additional strategies to enhance the vaccine potency.
Methods: We tested the HPV-16 E7 peptide-based vaccine in combination with a strategy to enhance CD4+ T
help using a Pan HLA-DR epitope (PADRE) peptide and a strategy to enhance dendritic cell activation using the
toll-like receptor 3 ligand, poly(I:C).
Results: We observed that mice vaccinated with E7 peptide-based vaccine in combination with PADRE peptide
and poly(I:C) generated better E7-specific CD8+ T cell immune responses as well as significantly improved
therapeutic anti-tumor effects against TC-1 tumors compared to E7 peptide-based vaccine with either PADRE
peptide or poly(I:C) alone. Furthermore, we found that intratumoral vaccination with the E7 peptide in conjunction
with PADRE peptide and poly(I:C) generates a significantly higher frequency of E7-specific CD8+ T cells as well as
better survival compared to subcutaneous vaccination with the same regimen in treated mice.
Conclusions: The combination of PADRE peptide and poly(I:C) with antigenic peptide is capable of generating
potent antigen-specific CD8+ T cell immune responses and antitumor effects in vaccinated mice. Our study has
significant clinical implications for peptide-based vaccination. - 中文關鍵字: --
- 英文關鍵字: --