- 作者: Nobuhito Fukaya; Lu-Ping Chow; Yoshitsugu Sugiura; Akira Tsugita; Yoshio Ueno; Kiyoshi Tabuchi
- 作者服務機構: a Department of Microbiology I, School of Veterinary Medicine, Azabu University, Kanagawa, b Department of Toxicology and Microbial Chemistry, Faculty of Pharmaceutical Sciences, Science University of Tokyo, Tokyo, c Research Institute for Biosciences, Science University of Tokyo, Noda, Japan
- 中文摘要: --
- 英文摘要: Peptidyl-proprylyl cis-trans isomerase (PPIase) activity was observed from crude extract of Fusarium sporotrichioides. Proteins from this fungi were separated by two-dimensional polyacrylamide gel electrophoresis and more than one thousand protein spots were separated. Two cytosolic PPIases were found by the N-terminal sequencing from the two separated spots. The N-terminal 41 residues of the major protein spot showed high sequence identity (63.4%) with PPIase from Neurospora crassa. This protein was designated as PPIase a, having an apparent molecular mass of 20 kD and pI 7.0. The minor other protein spot, having a similar molecular mass but distinguishable pI 6.4, was also sequenced and the N-terminal twenty residues were almost identical to PPIase a and was designated as PPIase b.
- 中文關鍵字: --
- 英文關鍵字: Fusarium sporotrichioides ; Peptidyl-prolyl isomerase ; Partial amino acid sequence