• 作者： 王三郎; 謝順堂; 白其昇
• 作者服務機構： 大葉工學院食品工程系
• 中文摘要： 由細菌M-1001之發酵上清液分離出兩種溶菌?抑制劑F-I及F-II。此菌株經鑑定係為Pseudomonas aeruginosa。於含
  0.25％葡萄糖，0.25％牛肉萃取物，0.25％蛋白?，1.0％麩胺酸鈉，以及1.0％可溶性澱粉（pH 7.0）之培養基中，於
  37°C培養20-24小時可得較佳之抑制活性。取P. aeruginosa M-1001培養上清液，經硫酸銨沈澱，再以DEAE-Sepharose
  CL-6B陰離子交換層析及Sephacryl S-200膠體過濾層析進行抑制劑之分離，可純化出F-I及F-II兩種具抑制活性之蛋白
  質，F-I及F-II之純度分別提昇了20及7.5倍，分子量分別約為57,000及33,000道耳頓，pH安定性分別為6-10及6-11，熱安
  定性分別為50°C及40°C。P. aeruginosa M-1001所生產抑制劑對測試之革蘭氏陽性菌具抑制效果。
  a
• 英文摘要： Two proteinaceous lysozyme inhibitors, hen-egg-white lysozyme inhibitors F-I and F-II, were isolatedfrom the culture broth of a bacterial strain identified as Pseudomonas aeruginosa M-1001. Maximumlysozyme inhibitory activity was obtained when the bacterium was grown aerobically in a medium consistingof 0.25％ glucose, 0.25％ beef extract, 0.25％ polypepton, 1.0% sodium L-glutamate, and 1.0% solublestarch (pH 7.0) at 37°C after 20-24 hrs. F-I and F-II were purified 20 and 7.5-fold, respectively, from theculture supernatant of P. aeruginosa M-1001 by ammonium sulfate fractionation, DEAE-Sepharose CL-6Bcolumn chromatography, and Sephacryl S-200 gel chromatography. The molecular weights of F-I and F-IIwere estimated to be about 57,000 and 33,000, by SDS-PAGE, respectively. F-I was stable in a pH rangebetween 6 and 10 and below 50°C. F-II was stable in a pH range between 6 and 11 and below 40°C.Many Gram-positive bacteria were found to be inhibited by the crude lysozyme inhibitors.
• 中文關鍵字： lysozyme; lysozyme inhibitor; antibiotic; Pseudomonas aeruginosa.
• 英文關鍵字： --