- 作者: 沈佩明 ; 蕭明熙 ; 鍾慧茹 等
- 作者服務機構: 榮民總醫院教學研究部
- 中文摘要: Reversed-phase high-performance liquid chromatography(RP-HPLC) was used as a tool to explore the retention behavior and separation of four 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors, namely compactin, lovastatin, lovastatin, simvastatin, and pravastatin in their hydroxy acid and lactone forms. The contribution of C- 6 and C-2' methyl groups and lactonization to the molecular hydrophobicity among these four structurally related HMG- CoA reductase inhibitors were elucidated. Eight components(four lactones and four hydroxy acids) could be resolved by RP-HPLC with isocratic elution. In a binary mobile phase system of acetonitrile-water containing 0.5% acetic acid, the free hydroxy acids and corresponding lactone forms remained intact and were completely separated. This study demonstrated that RP-HPLC is suitable for simultaneous determination of active and prodrug forms of these HMG-CoA reductase inhibitors.
- 英文摘要: --
- 中文關鍵字: Liquid Chromatography; Reversed Phase; High Performance Liquid Chromatography (Hplc); Compactin; Lovastatin; Pravastatin
- 英文關鍵字: 液相層析;逆相;高效能液相層析