- 作者: Paulina M. Kowalewska, Uyen T. Nguyen, Lori L. Burrows and Alison E. Fox-Robichaud
- 作者服務機構: Thrombosis and Atherosclerosis Research Institute and the Department of Medicine, McMaster University, Hamilton, ON, Canada
- 中文摘要: --
- 英文摘要:
Background:
Technique failure in peritoneal dialysis (PD) due to fibrosis and angiogenesis is complicated by
peritonitis. Staphylococcus aureus infection is one of the most common causes of peritonitis in PD. The heparan
sulfate proteoglycan, syndecan-1 (CD138), was reported to regulate fibrosis, angiogenesis, inflammation and S.
aureus infection. The objectives of this study were to examine the effects of syndecan-1 on S. aureus infection
and histopathology in a PD model.
Results:
Syndecan-1-/- and wild type mice were dialyzed for 4 weeks and infected intraperitoneally with S. aureus.
Tissues were collected after 4 h for histomorphometric analysis. Intravital microscopy was used to observe leukocyte
recruitment and to quantify syndecan-1 in the parietal peritoneum microcirculation. The dialyzed syndecan-1-/-
mice were more susceptible to S. aureus infection than undialyzed syndecan-1-/- controls and wild type animals.
However, peritoneal fibrosis and neovascularization due to PD did not differ between syndecan-1-/- and wild type
mice. Intravital microscopy showed that in S. aureus infection, syndecan-1 was removed from the subendothelial
layer of peritoneal venules but syndecan-1 deficiency did not affect leukocyte recruitment.
Conclusions:
This study indicates that, while syndecan-1 is important for providing a barrier to acute S. aureus
infection in PD, it does not affect peritoneal fibrosis and angiogenesis. - 中文關鍵字: --
- 英文關鍵字: Intravital microscopy, Leukocyte recruitment, Microcirculation, Fibrosis, Peritonitis, Proteoglycans