- 作者: Jen-Chuang Kung; Tsung-Chieh Chen; Bai-Chuang Shyu; Sigmund Hsiao; Andrew Chih Wei Huang
- 作者服務機構: Institute of Biomedical Sciences, Academia Sinica, Taiwan, Republic of China
- 中文摘要: --
- 英文摘要:
The present study used the preproenkephalin knockout (ppENK) mice to test
whether the endogenous enkephalins deficit could facilitate the anxiety- and
depressive-like symptoms of posttraumatic stress disorder (PTSD). On Day 1, sixteen
wildtype (WT) and sixteen ppENK male mice were given a 3 mA or no footshock
treatment for 10 seconds in the footshock apparatus, respectively. On Days 2, 7, and
13, all mice were given situational reminders for 1 min per trial, and the freezing
response was assessed. On Day 14, all mice were tested in the open field test, elevated
plus maze, light/dark avoidance test, and forced swim test. Two hours after the last
test, brain tissues were stained to examine c-fos expression in specific brain areas. The
present results showed that the conditioned freezing response was significant for
different genotypes (ppENK vs WT). The conditioned freezing effect of the ppENK
mice was stronger than those of the WT mice. On Day 14, the ppENK mice showed
more anxiety- and depressive-like responses than WT mice. The magnitude of Fos
immunolabeling was also significantly greater in the primary motor cortex, bed
nucleus of the stria terminalis-lateral division, bed nucleus of the stria
terminalis-supracapsular division, paraventricular hypothalamic nucleus-lateral
magnocellular part, central nucleus of the amygdala, and basolateral nucleus of the
amygdala in ppENK mice compared with WT mice. In summary, animals with an
endogenous deficit in enkephalins might be more sensitive to PTSD-like aversive
stimuli and elicit stronger anxiety and depressive PTSD symptoms, suggesting an
oversensitivity hypothesis of enkephalin deficit-induced PTSD. - 中文關鍵字: --
- 英文關鍵字: --