- 作者: Nicholette B. Larsen; Harry W. Kestler; John J. Docherty
- 作者服務機構: a Department of Microbiology/Immunology, Northeastern Ohio Universities College of Medicine, Rootstown, Ohio, and; b Department of Mathematics and Science, Lorain County Community College, Lorain, Ohio, USA
- 中文摘要: --
- 英文摘要: An intact nef gene is essential for rapid development of immunodeficiency in human immunodeficiency virus and simian immunodeficiency virus infections. To assess the role of nef in the immune response, mice transgenic for SIV nef were constructed and the humoral and cellular immune response to herpes simplex virus type-1 (HSV-1), measured. Mice transgenic for SIV-mac239 nef exhibited a significantly increased mortality rate when challenged with HSV-1 and also showed unusual antibody kinetics in response to viral challenge. During a 32-week period following exposure to HSV, it was noted that IgG subclass titers continued to rise in the nef+ animals, while titers of nef- animals decreased. Additionally, following secondary challenge with HSV, nef- mice had a significantly greater rise in HSV-neutralizing antibody titers than nef+ mice. A decreased proliferative response to the T cell mitogen, PHA, was noted in the nef+ animals. These results suggest that the presence of nef+ is sufficient to induce immune dysfunction.
- 中文關鍵字: --
- 英文關鍵字: Simian immunodeficiency virus; nef; Transgenic mice ; Herpes simplex virus ; IgG1 ; IgG2a