- 作者: Camli, Ugur ; Murray, T. ; Aguayo, J. et al.
- 作者服務機構: Dept. of Environmental Health Sciences, Johns Hopkins Univ., Baltimore, Maryland, U.S.A.
- 中文摘要: The sorbitol theory in diabetic cataractogenesis was based on sorbitol accumulation under glucose stress. Sorbitol accumulation was examined by /sup 13/C nuclear magnetic resonance spectroscopy (NMR) for the first time in matched human lenses incubated in 5.5 mM and 35.5 mM C-1/sup 13/C-enriched glucose up to 28 hours. The results showed that sorbitol and lactate in human lens can be detected at 35.5 mM, but not in 5.5 mM glucose solution. The glycolysis metabolic pathway of human lenses may be quite different from that of animals. The accumulation of metabolites can be traced and quantified by the intensities of /sup 13/C NMR peaks. Therefore, /sup 13/C NMR spectroscopy can be used as a valuable tool to investigate human lens carbohydrate metabolism non-interventionally.
- 英文摘要: --
- 中文關鍵字: Human Lenses; Glucose Stress; Sorbitol; Lactate; Diabetic Cataractogenesis; Carbon-13 Nuclear Magnetic Resonance
- 英文關鍵字: 碳13核磁共振;人的水晶體;山梨醇;乳酸鹽;糖尿病引發之白內障