- 作者: Junkun He Curtis G. Hayes Leonard N. Binn Jitvimol Seriwatana David W. Vaughn Robert A. Kuschner Bruce L. Innis
- 作者服務機構: Department of virus Diseases, Walter Reed Army Institute of reasearch, Waltter Reed Army Medical Center and Infectious Dlseases Directorate, Naval Medical Research Center, Silver Sping, Md., USA
- 中文摘要: --
- 英文摘要: Injection of an expression vector pJHEV containing hep-atitis E virus (HEV) structural protein open reading frame2 gene generates a strong antibody response in BALB/cmice that can bind to and agglutinate HEV. In this study,we tested for immunologic memory in immunized micewhose current levels of IgG to HEV were low or undetect-able despite 3 doses of HEV DNA vaccine 18 months ear-lier. Mice previously vaccinated with vector alone werecontrols.All mice were administered a dose of HEV DNAvaccine to simulate an infectious challenge with HEV.The endpoint was IgG to HEV determined by ELISA. Tendays after the vaccine dose, 5 of 9 mice previously immu-nized with HEV DNA vaccine had a slight increase in IgGto HEV. By 40 days after the vaccine dose, the level of IgGto HEV had increased dramatically in all 9 mice (108-foldincrease in geometric mean titer). In contrast, no controlmice became seropositive. These results indicate thatmice vaccinated with 3 doses of HEV DNA vaccine retainimmunologic memory. In response to a small antigenicchallenge delivered as DNA, possibly less than deliveredby a human infective dose of virus, mice with memorywere able to generate high levels of antibody in less timethan the usual incubation period of hepatitis E. We spec-ulate that this type of response could protect a humanfrom overt disease.
- 中文關鍵字: --
- 英文關鍵字: DNA vaccine. Hepatitls E virus. Memory, immunologic