• 作者： 陳瑞山; 侯豐男
• 作者服務機構： 國立中興大學昆蟲學研究所
• 中文摘要： 木省所發生之家蠶核多角體病毒，以光學顯微鏡觀察，其包含體形狀為六面體，少數為四面體；直徑約1.5～4.0μ。罹病初期的家蠶並無明顯的病徵，至末期體節腫大，表皮呈白色，並且極為脆弱；破裂後則流出牛奶狀之白濁體液－膿汁。病蠶解剖後中腸呈混濁白色，與健蠶之綠色者，迥然而異。三齡之家蠶幼蟲接種多角體病毒後，用石蠟切片法觀察各組織細胞，以氣管皮膜組織、脂肪體細胞、體壁表皮層細胞、血球細胞最易受感染；中腸上皮層細胞、馬氏管、絲腺、神經鞘細胞、肌肉鞘細胞也微有感染之現象；肌纖維、背管則不含核多角體病毒。具感受性之組織在接種後48或72小時，細胞核有膨大之現象，係病毒在此複製之結果。
• 英文摘要： The inclusion bodies of nclear polyhedrosisvirus (NPV) of the silkworm, Bombyx mori L.,reared in Taiwan were observed with lightmicroscopy. The polyhedra were hexagonalor tetragonal with 1.5-4.0μ in diameter. The diseased larvae showed no apparentsymptom at the initial stage of infection. Butthey lost appetite and the intersegmentalmembranes were swollen at the advanced stage.Their integument became very fragile andemitted a milky white fluid when ruptured. When the diseased larvae were dissected,the midgut was opaque and milky white inappearance as compared to the green midgut ofhealthy larvae. The tracheal matrix, fat body cells,epidermal cells and hemocytcs were heavilyinfected sites. Midgut cells, Malpighian tubules,silk glands, nerve sheath and muscle sheathwere slightly infected, while muscle fibrils anddorsal vessel were free of NPV infection. Thenuclei of susceptible cells showed swollen at48 or 72 hr after inoculation, indicatingreplication of the virus at this cellular structure.
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