- 作者: Ahmed S Abdel-Moneim; Ahmad E Abdel-Ghany; Salama AS Shany
- 作者服務機構: Department of Virology, Faculty of Veterinary Medicine, Beni-Suef University, Egypt
- 中文摘要: --
- 英文摘要:
BACKGROUND: The highly pathogenic H5N1 is a major avian pathogen that crosses species
barriers and seriously affects humans as well as some mammals. It mutates in an intensified
manner and is considered a potential candidate for the possible next pandemic with all the
catastrophic consequences.
METHODS: Nasal swabs were collected from donkeys suffered from respiratory distress. The
virus was isolated from the pooled nasal swabs in specific pathogen free embryonated
chicken eggs (SPF-ECE). Reverse transcriptase polymerase chain reaction (RT-PCR) and
sequencing of both haemagglutingin and neuraminidase were performed. H5
seroconversion was screened using haemagglutination inhibition (HI) assay on 105 donkey
serum samples.
RESULTS: We demonstrated that H5N1 jumped from poultry to another mammalian host;
donkeys. Phylogenetic analysis showed that the virus clustered within the lineage of H5N1
from Egypt, closely related to 2009 isolates. It harboured few genetic changes compared to
the closely related viruses from avian and humans. The neuraminidase lacks oseltamivir
resistant mutations. Interestingly, HI screening for antibodies to H5 haemagglutinins in
donkeys revealed high exposure rate.
CONCLUSIONS: These findings extend the host range of the H5N1 influenza virus, possess
implications for influenza virus epidemiology and highlight the need for the systematic
surveillance of H5N1 in animals in the vicinity of backyard poultry units especially in endemic
areas. - 中文關鍵字: --
- 英文關鍵字: --