- 作者: Bor-Ru Lin, Chia-Jung Yu, Wang-Chuan Chen, Hsuan-Shu Lee, Huei-Min Chang, Yen-Chih Lee, Chiang-Ting Chien, Chau-Fong Chen
- 作者服務機構: 1Graduate Institute of Physiology, National Taiwan University College of Medicine, Taipei, R.O.C.
- 中文摘要: --
- 英文摘要:
Oxidative stress and inflammation contributed to the propagation of acute liver injury (ALI).
The present study was undertaken to determine whether D-galactosamine (D-GalN) induces
ALI via the mitochondrial apoptosis- and proinflammatory cytokine-signaling pathways, and
possible mechanism(s) by which green tea (GT) extract modulates the apoptotic and
proinflammatory signaling in rat. D-GalN induced hepatic hypoxia/hypoperfusion and
triggered reactive oxygen species (ROS) production from affected hepatocytes, infiltrated
leukocytes, and activated Kupffer cells. D-GalN evoked cytosolic Bax and mitochondrial
cytochrome C translocation and activated proinflammatory nuclear factor-kappa B (NF-κB)
and activator protein-1 (AP-1) translocation, contributing to the increase of intercellular
adhesion molecule-1 expression, terminal deoxynucleotidyl transferase-mediated nick-end
labeling (TUNEL)-positive hepatocytes, multiple plasma cytokines and chemokines release,
and alanine aminotransferase (ALT) activity. An altered biliary secretion profile of several
acute phase proteins directly indicates oxidative stress affecting intracellular trafficking in
the hepatocyte. GT pretreatment attenuated ROS production, mitochondrial apoptosis- and
proinflammatory cytokine-signaling pathway, plasma ALT and cytokines levels, biliary acute
phase proteins secretion and hepatic pathology by the enhancement of anti-apoptotic
mechanisms. In conclusion, D-GalN induced ALI via hypoxia/hypoperfusion-enhanced
mitochondrial apoptosis- and proinflammatory cytokine-signaling pathway, contributing to
oxidative stress and inflammation in the liver. GT can counteract the D-GalN-induced ALI
via the attenuation of apoptotic and proinflammatory signaling by the upregulation of
anti-apoptotic mechanism. - 中文關鍵字: --
- 英文關鍵字: --