- 作者: Yung-Chin Hsiao; Yih-Shou Hsieh; Wu-Hsien Kuo; Hui-Ling Chiou; Shun-Fa Yang; Whei-Ling Chiang; Shu-Chen Chu
- 作者服務機構: 1 Institute of Biochemistry and Biotechnology, Chung Shan Medical University, No 110, Section 1, Chien Kuo N. Road, Taichung 402, Taiwan; ; 2 Division of Gastroenterology, Department of Internal Medicine, Armed-Force Taichung General Hospital, Taiping City, Taichung 411, Taiwan; ; 3 School of Medical Laboratory and Biotechnology, Chung Shan Medical University, No 110, Section 1, Chien Kuo N. Road, Taichung 402, Taiwan; ; 4 Institute of Medicine, Chung Shan Medical University, No 110, Section 1, Chien Kuo N. Road, Taichung 402, Taiwan; 5 Department of Food Science, Central Taiwan University of Science and Technology, No. 11 Pu-tzu Lane, Pu-tzu Road, Taichung 406, Taiwan; ; 6 General Education Center, Central Taiwan University of Science and Technology, No.11 Pu-tzu Lane, Pu-tzu Road, Taichung 406, Taiwan
- 中文摘要: --
- 英文摘要: Natural products, including flavonoids, are suggested to be involved in the protective effects of fruits and vegetables against cancer. However, studies concerning the effect of flavonoids frequently lacked data regarding to flavanones. In this study, we investigated the inhibitory effect of flavanone compounds, including flavanone, 2'-OH flavanone, 4'-OH flavanone, 6-OH flavanone, naringin and naringenin, on cell growth of various cancer cells. We determined that flavanone and 2'-OH flavanone inhibited cell growth of A549, LLC, AGS, SK-Hepl and HA22T cancer cells, while other flavanones showed little or no inhibition. We evaluated growth-inhibitory activity of flavanone and 2'-OH flavanone against highly proliferative human lung cancer cells (A549) via anchorage-independent and -dependent colony formation assay, and further showed that treatment of flavanone resulted in a Gl cell cycle arrest with reduction of cyclin D, E and cyclin-dependent kinase (CDK) 2, while treatment of 2'-OH flavanone led to a G2/M phase accumulation with reduction of cyclin B, D and Cdc2. Moreover, we demonstrated the improvement effect of flavanone and 2'-OH flavanone with anti-cancer drug, doxorubicin, on A549 cells. Finally, flavanone and 2'-OH flavanone were evidenced by its inhibition on the growth of A549 and Lewis lung carcinoma cells in vivo.
- 中文關鍵字: --
- 英文關鍵字: flavanone compounds, A549 human lung cancer cells, cyclin-CDK complex