- 作者: Hongxin Gui; Chi-Wei Lu; Sandra Adams; Victor Stollar; Mei-Ling Li
- 作者服務機構: Department of Physiology & Biophysics, Molecular Genetics, Microbiology & Immunology, UMDNJ-Robert Wood Johnson Medical School, Piscataway NJ, USA
- 中文摘要: --
- 英文摘要:
Background: Sindbis virus (SV) is the prototype of alphaviruses which are a group of widely distributed human and animal pathogens. Heterogeneous nuclear
ribonucleoprotein (hnRNP) A1 is an RNA-binding protein that shuttles between
the nucleus and the cytoplasm. Our recent studies found that hnRNP A1
relocates from nucleus to cytoplasm in Sindbis virus (SV)-infected cells. hnRNP
A1 binds to the 5’ UTR of SV RNA and facilitates the viral RNA replication and
translation.
Methods: Making use of standard molecular techniques, virology methods and an in vitro
system developed by our lab to assess the role of hnRNP A1 in SV positive
strand RNA synthesis.
Results: hnRNP A1 interacted with the genomic (G) and subgenomic (SG) RNA
promoters. Knockdown of hnRNP A1 resulted in markedly decrease in the
synthesis of G and SG RNA both in infected cells and in vitro.
Conclusions: Our study provides the first direct evidence that hnRNP A1 actively participates in
viral RNA replication and is required for the synthesis of G and SG RNA. - 中文關鍵字: --
- 英文關鍵字: --