• 作者： 陳宛珂; 王家弘; 李昭仁
• 作者服務機構： 清華大學化學工程研究所; 台北榮民總醫院呼吸治療科
• 中文摘要： 自人類和豬血液製備的純化（無抗原性）血紅素，以精密儀器如光掃描、電泳（SDS-PAGE、IEF）、及HPLC等分析鑑定。結果顯示人類和豬血紅素並無明顯的區別。純化之豬血紅素與人血間之適存性和功能則以下方式測試；因素活化、串級凝血、分解纖維蛋白法、及紅血球形態變化之電子顯微鏡觀測法。結果顯示豬血紅素之生物適合性一般說來良好，豬血紅素應可考慮為備製代用紅血球之天然原料。
• 英文摘要： Stroma-free hemoglobins (Hb) prepared from human and pig bloods were characterized by analyticalmethods including light scanning, electrophoresis (SDS-PAGE and IEF), and HPLC analysis. The resultsshowed no significant difference between human and pig hemoglobins. The compatibility and functionsof pig Hb in human blood were tested by using factor activation, coagulation cascade, the fibrinolyticprocess, and by observation of morphology changes of RBC under an electromicroscope. The resultsindicate that the biocompatibilities of pig Hb are generally acceptable; thus, pig Hb may be developedas a potential raw material for an erythrocyte substitute useful in emergency blood transfusion.
• 中文關鍵字： hemoglobin; isoelectric point; erythrocytes; blood substitute; RBC morphology
• 英文關鍵字： --