• 作者： 陳家福; 黃經民; 饒樹文; 呂福江; 陳光耀
• 作者服務機構： 國防醫學院醫學科學研究所及三軍總醫院醫學研究部; 三軍總醫院外科部大腸直腸外科; 三軍總醫院病理部
• 中文摘要： 利用新近發展出來之微囊成型技術，我們發展出一種具有適度通透性之半透膜空心球體。此種微囊對於分子量為45,-000及66,000的血清蛋白以及分子量為64,000的純化人類血紅素不具通透性，但對於低分子量的物質諸如阿爾法乳白蛋白（分子量為14,200）及胰蛋白酵素元（分子量為24,000）則可以通過。體內實驗結果顯示微囊包埋之癌細胞株（如KB，人類口腔上皮癌；P-388淋巴性白血癌；GBM8401/TSGH，腦神經膠質瘤）及人類大腸癌細胞在一段培養期內隨著時間而呈線性生長與繁殖。此種生長情況在體內培養優於體外培養。組織型態學上之檢查顯示此種微囊包埋之癌細胞株與未包埋之原始細胞無異。以三種已知之化療藥物（Adriamycin,　5-Fu, Cyclophosphamide）與包埋之癌細胞株行體外培養時發現它們對於包埋與未包埋之癌細胞株的抑制百分比無顯著差異。體內培養實驗結果顯示不同之抗癌藥物對同一癌細胞具不同之抑制效果。本研究結論“微囊包埋之癌細胞”模式是一理想抗癌藥物篩選方法，冀望能在不久之未來應用於臨床。
• 英文摘要： A microencapsulation of living tumor cells by an improved membrane and droplet forming techniquewas established in our laboratory. This semipermeable microencapsulating membrane was impermeableto serum albumins (M.W. 66,000 or 45,000) and human hemoglobin (M.W. 64,000), but permited passageof low molecular weight substances (α-Lactalbunun, or Trypsinogen; M.W. 14,200 or 24,000). The invivo results showed that microencapsulated tumor cell lines (KB, human oral epidermoid cell; P-388 lym-phocytic leukemia; GBM 8401/TSGH, glioma) and human colorectal carcinoma cells grew and proli-ferated exponentially within twenty days. The in vivo growth exhibited better than that in vitro. Histo-logical and morphological findings of these four different kinds of tumor cells are similar to those of or-iginal tumor cells. Treatment of the microencapsulated tumor cells (MTC) with cytotoxic drugs (adriamyc-in, 5-fluorouracil and cyclophosphamide) in vitro showed no significant difference in percent inhibition(p > 0.05) between the encapsulated and non-encapsulated cells. The in vivo data indicated that differentanti-cancer drugs had different inhibition effects. The results showed that the MTC model was useful forscreening an appropriate cytotoxic drug and could be applied to clinical medicine in the near future.
• 中文關鍵字： microencapsulation; tumor cells; screening of anticancer drugs
• 英文關鍵字： --