- 作者: Yi-Tsau Huang Yi-Chao Hsu Chi-Jen Chen Chien-Tzu Liu Yau-Huei Wei
- 作者服務機構: Institute of Traditional Medicine, School of Medicine, and Department of Biochemistry, School of Life Science, Batuibak Yang-Ming University, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要:
The role of reactive oxygen species in liver fibrogenesis
is not yet clarified. The aim of this study was to investi-
gate oxidative-stress-related changes in cirrhotic rats.
Cirrhosis was induced by bile duct ligation in sprague-
Dawley rats. Plasma malondialdehyde (MDA), hepatic 8-
hydroxy-2'-deoxyguanosine (8-OHdG), hepatic mito-
chondrial respiratory functions and gene transcripts
were measured at 2 and 4 weeks after surgery in bile-
duct-ligated (BDL) and sham-operated-operated rats.
The results showed progressive increases in the levels of
plasma MDA, hepatic 8-OHdG and procollagen I and III
mRNA expression, and progressive impairment of he-
patic mitochondrial respiratory function in BDL rats at 2
and 4 weeks after ligation compared with sham-operated
rats. Moreover, at 4 weeks after ligation, BDL rats exhib-
ited reduced plasma glutathione and vitamin E levels,
impaired hepatic mitochondrial electron transport en-
zyme activities and oxidative phosphorylation function.
In addition, hepatic mRNA expression of transforming
growth factor-β1 was increased. Hepatomegaly, abnor-
mal plasma alanine transaminase and aspartate trans-
aminase levels, and portal hypertension were noted in
BDL rats. Our results suggest that bile duct ligation in the
rat induces mitochondrial dysfunction and biocheical
and molecular changes related to oxidative stress in the
liver. - 中文關鍵字: --
- 英文關鍵字: Fibrosis.Malondialdehyde. 8-Hydroxy-2-deoxyguanosine. Transforming growth factor-β1. Collagen. Mitochondrial electron transport chain