- 作者: Kuo-Feng Tai; Ding-Shinn Chen; Lih-Hwa Hwang
- 作者服務機構: Graduate Institute of Microbiology and Hepatitis Research Center, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要: In preclinical studies, tumor cells genetically engineeredto secrete cytokines, hereafter rferred to as tumor cellvaccines, can often generate systemic antitumor immu-nity. This study investigated the therapeutic effects oflive or irradiated tumor cell vaccines that secrete granu-locyte-macrophage colony-stimulating factor(GM-CSF)on established orthotopic liver tumors. Experimental re-sults indicated that two doses (3 x 107 cells per dose)ofirradiated tumor cell vaccines were therapeutically inef-fective, whereas one dose(3 x 106 cells) of live tumorcell vaccines caused complete tumor regression. In vivodepletion of CD8+ T cells, but not natural killer cells,restored tumor formation in the live vaccine-treated ani-mals. Additionally, the treatment of cells with live vac-cine induced markedly higher levels of cytotoxic T lym-phocyte activity than the irradiated vaccines in the drain-ing lymph nodes.The higher levels of cytokine and anti-gen loads could partly explain the superior antitumoractivity of live tumor cell vaccines, but other unidentifiedmechanisms could also play a role in the early T cell acti-vation in the lymph nodes. A protocol using multiple andhigher dosages of irradiated tumor cell vaccines alsocaused significant regression of liver tumors.These re-sults suggest that the GM-CSF-secreting tumor cell vac-cines are highly promising for orthotopic liver tumors ifhigher levels of immune responses are elicited duringearly tumor development.
- 中文關鍵字: --
- 英文關鍵字: --