- 作者: Bhanu Iyengar; Avantika V Singh
- 作者服務機構: Histochemistry Department, Institute of Pathology, New Delhi, India
- 中文摘要: --
- 英文摘要:
Background: Melanomas, highly malignant tumors arise from the melanocytes which originate
as multipotent neural crest cells during neural tube genesis. The purpose of this study is to
assess the pattern of neural differentiation in relation to angiogenesis in VGP melanomas using
the tumor as a three dimensional system.
Methods: Tumor-vascular complexes [TVC] are formed at the tumor-stroma interphase, by
tumor cells ensheathing angiogenic vessels to proliferate into a mantle of 5 to 6 layers [L1 to L5]
forming a perivascular mantle zone [PMZ]. The pattern of neural differentiation is assessed by
immunopositivity for HMB45, GFAP, NFP and synaptophysin has been compared in: [a] the
general tumor [b] tumor-vascular complexes and [c] perimantle zone [PC] on serial frozen and
paraffin sections. Statistical Analysis: ANOVA: Kruskal-Wallis One Way Analysis of Variance; All
Pairwise Multiple Comparison Procedures [Tukey Test].
Results: The cells abutting on the basement membrane acquire GFAP positivity and extend
processes. New layers of tumor cells show a transition between L2 to L3 followed by NFP and
Syn positivity in L4&L5. The level of GFAP+vity in L1&L2 directly proportionate to the percentage
of NFP/Syn+vity in L4&L5, on comparing pigmented PMZ with poorly pigmented PMZ. Tumor
cells in the perimantle zone show high NFP [65%] and Syn [35.4%] positivity with very low GFAP
[6.9%] correlating with the positivity in the outer layers.
Discussion: From this study it is seen that melanoma cells revert to the embryonic pattern of
differentiation, with radial glial like cells [GFAP+ve] which further differentiate into neuronal
positive cells [NFP&Syn+ve] during angiogenic tumor-vascular interaction, as seen during
neurogenesis, to populate the tumor substance. - 中文關鍵字: --
- 英文關鍵字: --