• 作者： 謝國珍; 陳慧玲; 陳豪勇; 洪其璧
• 作者服務機構： 行政院衛生署預防醫學研究所病毒組
• 中文摘要： 本研究是利用聚合?鏈鎖反應，來偵測急性期病人淋巴球之恙蟲病立克次體DNA。所採用的primer，是針對恙蟲
  病立克次體56 kDa之抗原基因的特定DNA序列。其DNA產物為78 bp DNA，此DNA產物可與標準株Gilliam之DNA探
  針雜合（hybride）。另外比較聚合?鏈鎖反應、間接螢光偵測法及細胞培養法的偵測結果，顯示聚合?鏈鎖反應是在急性
  期偵測恙蟲病較好的偵測方法。
  U
• 英文摘要： Polymerase chain reaction was used to detect Rickettsia tsutsugamushi specific DNA from the lym-phocytes of patients in the acute phase of the disease. The primers used in the PCR were based on thenucleotide sequences of gene encoding of the 56 kDa antigen of the Gilliam strain. The PCR product isa 78 by fragment which can be hybridized by the 78 by DNA probe of the Gilliam strain. Comparisonof the results obtained by polymerase chain reaction, immunofluorescent assay and cell culture suggests thatthe polymerase chain reaction method is the most sensitive one among the three in diagnosis of scrub typhus.
• 中文關鍵字： Rickettsia tsutsugamushi; lymphocytes; acute phase; polymerase chain reaction.
• 英文關鍵字： --