- 作者: Ying-Ju Lin; Jia-Guan Peng; Suh-Chin Wu
- 作者服務機構: Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.
- 中文摘要: --
- 英文摘要:
The interaction between prM and E proteins in flavivirus-infected cells is a major
driving force for the assembly of flavivirus particles. We used site-directed
mutagenesis to study the potential role of the transmembrane domains of the prM
proteins of Japanese encephalitis virus (JEV) in prM-E heterodimerization as well as
subviral particle formation. Alanine insertion scanning mutagenesis within the
GXXXG motif in the first transmembrane segment of JEV prM protein affected the
prM-E heterodimerization; its specificity was confirmed by replacing the two glycines
of the GXXXG motif with alanine, leucine and valine. The GXXXG motif was found
to be conserved in the JEV serocomplex viruses but not other flavivirus groups. These
mutants with alanine inserted in the two prM transmembrane segments all impaired
subviral particle formation in cell cultures. The prM transmembrane domains of JEV
may play importation roles in prM-E heterodimerization and viral particle assembly. - 中文關鍵字: --
- 英文關鍵字: --