• 作者： 周德源
• 作者服務機構： 中央研究院植物研究所
• 中文摘要： 會萌芽和已喪失萌芽力的豌豆種子，在發芽的浸潤時期都可以吸取外加的放射性先質“可萌芽的種胚在浸潤作用開始第一小時內即開始有合成蛋白質及RNA的活力“DNA的合成要在萌芽八小時以後才開始進行。不會萌芽的種胚既不合成蛋白質也不合成DNA，但仍然保有相當程度的RNA合成能力。雖然蛋白質的合成不能在已喪失萌芽力的種胚裹面（in vivo)被測到，在試管內利用無細胞系統以合成聚合苯丙氨酸的試驗，發現尚未浸水的無萌芽力之種胚具有合成蛋白質的潛力。這種合成蛋白質的潛力在萌芽初期的浸潤時期遭受破壞。可能這些種胚不能合成蛋白質，成為不能萌芽的主要原因之
• 英文摘要： Both viable and non-viable pea embryos took up the radioactive precursorsduring imbibition. Viable embryotic axes begin to synthesize both protein andRNA as early as the first hour of imbibition. DNA synthesis in viable embryosdoes not commence until after 8 hours of germination. Non-viable embryos synthe-size neither protein nor DNA but retain a limited capacity for RNA synthesis.Although protein synthesis could not be detected in non-viable embryos in vivoduring early germination, experiments in vitro using a cell-free system to synthesizepolyphenylalanine suggest that the dry non-viable embryos possess a potentiallyfully active protein-synthesizing system·Ribosomes isolated from either dry viableor non-viable pea embryos were equally effective in the support of polyphenylal－anine synthesis in the cell-free system·Ribosomes isolated from imbibed non-viable axis tissue were impaired in their ability to support polyphenylalanine syn-thesis. The level of activity of phenylalaninyl-tRNA synthetase in the post-ribosomal supernatant fractions declined during dry storage and subsequent imbibi-tion of water by non-viable embryos．This indicates that imbibition of water bynon-viable axis tissue has a detrimental effect on the integrity of the protein syn-thetic apparatus of this tissue.
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