- 作者: Bernhard Aigner; Birgit Rathkolb; Martina Klempt; Sibylle Wagner; Dian Michel; Martin Hrabe de Angelis; Eckhard Wolf
- 作者服務機構: Department of Veterinary Sciences, and Laboratory for Functional Genome Analysis (LAFUGA), Gene Center, LMU Munich, Munich, Germany
- 中文摘要: --
- 英文摘要:
Background :
Clinical chemical blood analysis including plasma electrolytes is routinely carried out for the
diagnosis of various organ diseases. Phenotype-driven N-ethyl-N-nitrosourea (ENU) mouse
mutagenesis projects used plasma electrolytes as parameters for the generation of novel
animal models for human diseases.
Methods :
Here, we retrospectively evaluated the use of the plasma electrolytes calcium, chloride,
inorganic phosphorus, potassium and sodium in the Munich ENU mouse mutagenesis project
where clinical chemical blood analysis was carried out on more than 20,000 G1 and G3
offspring of chemically mutagenized inbred C3H mice to detect dominant and recessive
mutations leading to deviations in various plasma parameter levels.
Results :
We identified a small number of animals consistently exhibiting altered plasma electrolyte
values. Transmission of the phenotypic deviations to the subsequent generations led to the
successful establishment of mutant lines for the parameters calcium and potassium. Published
data from other phenotype-driven ENU projects also included only a small number of mutant
lines which were generated according to altered plasma electrolyte levels.
Conclusions :
Thus, use of plasma electrolytes detected few mouse mutants in ENU projects compared to
other clinical chemical blood parameters. - 中文關鍵字: --
- 英文關鍵字: --