- 作者: Habibllah Khajehsharifi* and Eslam Pourbasheer
- 作者服務機構: Department of Chemistry, Yasouj University, P. O. Box 75914-353, Yasouj, Iran
- 中文摘要: Genetic algorithm (GA) is a suitable method for selecting wavelengths for partial least squares (PLS) calibration of mixtures with almost identical spectra without loss of prediction capacity using the spectrophotometric method. In this study, the concentration model is based on absorption spectra in the range of 200-320 nm for 25 different mixtures of ascorbic acid (AA) and uric acid (UA). The calibration curve was linear over the concentration range of 1-15 and 2-16 μg mL-1 for ascorbic acid and uric acid, respectively. The root mean square deviation (RMSD) for ascorbic acid and uric acid with GA and without GA were 0.3071 and 0.3006, 0.3971 and 0.7063, respectively. The proposed method was successfully applied to the simultaneous determination of both analytes in human serum and urine samples.
- 英文摘要: --
- 中文關鍵字: Genetic algorithm; Spectrophotometric; Partial least squares; Ascorbic acid; Uric acid; Biological fluids.
- 英文關鍵字: --