- 作者: Yih-Loong Lai; Tai Chung Law
- 作者服務機構: Depatrment of Physiology, National Taiwan University College of Medicine, Taipei, Taiwan, ROC
- 中文摘要: --
- 英文摘要: A close relationship exists between hypoxia-induciblefactor(HIF)-1α and pulmonary hypertension. The presentstudy was carried out to explore if there are temporalalterations in HIF-1α levels during prolonged hypoxiaand after monocrotaline (MCT) treatment. First, youngWistar rats were divided into 5 groups: control, hyp-oxia-1, hypoxia-2, hypoxia-3 and hypoxia-4. Hypoxic ratswere placed in a closed hypobaric chamber (380 mm Hg)for a 1-week (hypoxia-1), 2-week (hypoxia-2), 3-week(hypoxia-3) or 5-week (hypoxia-4) period. Second, otheryoung Wistar rats were divided into 4 groups: control,MCT-1, MCT-2 and MCT-3. MCT-treated rats were in-jected subcutaneously once with MCT (60 mg/kg) for a1-week (MCT-1), 2-week (MCT-2) or 3-week (MCT-3) peri-od. Subsequently, pulmonary arterial pressure (Ppa) andthe weight ratio of the right ventricle to the left ventricleplus the septum [RV/(LV+S)] were measured, and lungswere obtained for the determination of HIF-1α via West-ern blot analysis. Both hypoxia and MCT induced tempo-ral increases in the Ppa, the ratio RV/(LV+S) and HIF-1αlevels. A close relationship between the Ppa and HIF-1αlevel was found in both hypoxia- and MCT-treated ani-mals. In addition, the Pa0 level significantly decreasedin rats 1-3 weeks after MCT treatment. These results,along with previous data in the literature, suggest thatboth chronic hypoxia- and MCT-induced lung hypoxiaactivate an increase in the production of HIF-1α, andresult in vascular remodeling and pulmonary hyperten-sion.
- 中文關鍵字: --
- 英文關鍵字: --