- 作者： 蘇遠志; 陳石麟
- 作者服務機構： 國立臺灣大學農業化學研究所
- 中文摘要：
構築Brevibacterium divaricatum基因庫於λEMBL3載體，該基因庫之力價為2.2×10 pfu/μg；利用噬菌體雜交
法挑出三隻含有完整色胺酸基因群之噬菌體，並從其中剪接出9.6 kb NcoI片段，此片段接於載體後，由於其能互補
trpA¯，trpC¯，trpE¯，甚至trpAE¯之不同色胺酸營養要求株，證明其含有完整之色胺酸基因群；聚合酵素連鎖反應
和去氧核醣限制酵素分析，發現B. divaricatum 色胺酸基因群亦以trpE，trpD，trp C，trpB ，trpA之次序排列存
在，表示棒狀桿菌之基因很類似，但彼此間仍有些許遺傳差異存在。
c - 英文摘要： A genomic bank from Brevibacterium divaricatum has been prepared using EMBL3 as a vector.The genomic bank's titers are 2.2 x 10 pfu/g. Through screening by plaque hybridization, a 9.6 kb NcoIfragment which contains the entire trp operon has been isolated. Polymerase chain reaction amplificationand restriction endonuclease analysis of PCR fragments indicated that there is homology between thecoryneform bacteria; however, some genetic diversity among the species still exists. By complementationtests using subcloning of the 9.6 kb NcoI fragments and various E. coli tryptophan auxotrophs, this frag-ment was found to contain a gene cluster composed of trpE, trpD, trpC, trpB and trpA in this order. Thisrevealed that the tryptophan biosynthesis genes in B. divaricatum may be an operon.
- 中文關鍵字： Brevibacterium divaricatum; genomic bank; trp operon; hybridization.
- 英文關鍵字： --
