- 作者: Mei-Ling Chang Yi-Ting Chen Yu-Chia Su John T. Kung
- 作者服務機構: Institute of Molecular Biology, Academia Sinica, Nakang District, and the Graduate Institute of Immunology, College of Medicine, Naotional Taiwan University, Taipei, Taiwan
- 中文摘要: --
- 英文摘要: P14 TCR transgenic CD8+ T cells(LCMV gp33-specific)were activated by antigen in the presence of either IL-2 orIL-2+IL-4 to generate effector cytotoxic T lymphocytes《CTLs).The therapeutic effectiveness of such IL-2- or IL-2+IL-4-grown CTLs was tested in mice that had receivedintravenous inoculations of B16.gp33 melanoma cells 7days previously. Administration of P14 CTLs activated byantigen+IL-2+IL-4 was significantly more effective atreducing melanoma colony formation in the lung thanthose grown in the presence of antigen+IL-2.Highly sig-nificant improvement in survival was observed with 80%of B16.gp33-inoculated mice showing long-term survivalafter therapy with 10 x 10 6 antigen+IL-2+IL-4-activatedP14 CTLs. Similar therapeutic effectiveness of antigen+IL-2+IL-4-activated P14 CTLs against subcutaneouslyinoculated B16.gp33 melanoma cells was also found.There was significant reduction in P14 CD8+T cells in theperipheral blood of B16.gp33-inoculated mice than inmice that did not receive B16.gp33 melanoma cells, indi-cating possible homing of P14 CD8+ T cells to the site oftumor growth or antigen-induced apoptotic cell death.These results may have implications in tumor therapyusing CTLs grown ex vivo, especially during early stagesof tumor formation.They also support the concept thatthe therapeutic effectiveness of CTLs can be governed bythe cytokine context in which they are activated.
- 中文關鍵字: --
- 英文關鍵字: Immunotherapy. IL-4. Adoptive transfer. CD8+ T cell. CTL. Melanoma. P14TCR