- 作者: 蔡玲美 ; 胡書銘 ; 王群等
- 作者服務機構: 中央研究院化學研究所
- 中文摘要: The secondary structure of the C-terminal fragment(10 kDa) of rat hsc70 (a.a. #546-646) was analyzed according to circular dichroism spectra. In the pH range 5- 9 and at concentrations of Na/sup +/ up to 0.18M, the protein is estimated to contain 32- 34% a helix, 18-16% of.beta. sheet, approximately 25% for both turns and disordered structures. This protein is most stable at pH between 6 and 7 in solution with 0.055M Na/sup +/ concentration. However, all T/sub m/ are relatively large(57-73.degree .C) and alterations from one condition to another are small. Thus, the C-fragment (10 kDa) of hsc70 is a stably folded protein under physiological conditions.
- 英文摘要: --
- 中文關鍵字: Heat-Shock Protein; C-Terminal Fragment; Circular Dichroism; Secondary Structure
- 英文關鍵字: 熱衝擊蛋白質;羧基端碎片;圓二分儀;二級結構