- 作者: Jing-Yi Lin, Jau-Shiuh Chen, Pei-Chun Chen, Ming-Hui Chung, Ching-Yi Liu, Shi-Chuen Miaw and Li-Fang Wang
- 作者服務機構: Department of Dermatology, Chang Gung Memorial Hospital, Chang Gung University College of Medicine, Taoyuan, Taiwan, R.O.C
- 中文摘要: --
- 英文摘要:
Background: Epicutaneous sensitization with protein allergen that induces predominant Th2 responses is
an important sensitization route in atopic dermatitis. Fungal components have been shown to
modulate Th cell differentiation. However, the effects of fungal components on epicutaneous
sensitization are unclear.
Results: In this study, we showed that co-administration of curdlan, a dectin-1 agonist, during
epicutaneous ovalbumin sensitization of BALB/c mice decreased the IL-5 and IL-13 levels in
supernatants of lymph node cell ovalbumin reactivation cultures. Mechanistically, curdlan coadministration
decreased IL-4 and IL-1β expressions in draining lymph nodes. Curdlan coadministration
also lower the migration of langerin+ CD103- epidermal Langerhans cells into
draining lymph nodes at 96 hours post-sensitization which might be attributed to decreased
expressions of IL-18 and IL-1β in patched skin. Moreover, adoptive transfer of CFSE-labeled
transgenic CD4 T cells confirmed that curdlan co-administration decreased the proliferation
and IL-4-production of ovalbumin -specific T cells primed by epidermal Langerhans cells.
Conclusions: These results indicated that concurrent exposure to a dectin-1 agonist suppresses the
epicutaneously induced Th2 response by modulating the cytokine expression profiles in
draining LNs and the migration of epidermal Langerhans cells. These results highlight the
effects of fungal components on epicutaneous allergen sensitization in atopic diseases. - 中文關鍵字: --
- 英文關鍵字: Fungus, Dectin-1, Epicutaneous sensitization, Th2 response