- 作者: 鄭建中,洪國展,皇甫維君
- 作者服務機構: Department of Chemistry and Graduate Institute of Life Sciences, Tamkang University, Taipei 251, Taiwan, R.O.C.
- 中文摘要: Single-stranded RNA molecules usually include secondary structural elements such as bulges, internal loops, and hairpin loops. These RNA secondary structural elements are often essential for the biological activity and functions of the RNA molecule. Chemical probe CoII(HAPP)(TFA)2 in the presence of H2O2 is found to differentiate single-stranded RNA from branched structures and hairpin loops. This study uses CoII(HAPP)(TFA)2 to analyze the structures of two RNA molecules: a fragment of HIV TAR RNA (TAR-27) and the catalytic domain 5 of group II intron (D5-29). The electrophoretic mobility of TAR-27 does not shift in the presence of CoII(HAPP)(TFA)2, suggesting that the reagent does not change the conformation of RNA substrate. Cleavage of the RNA substrates by CoII(HAPP)(TFA)2 unambiguously differentiated RNA internal looped structures from hairpin loops. The results show that CoII(HAPP)(TFA)2 is a sensitive, informative and convenient tool for analysis of RNA secondary structures.
- 英文摘要: --
- 中文關鍵字: Internal loop; Hairpin loop; Bulge; RNA cleavage; Cobalt.
- 英文關鍵字: --