• 作者： 劉仲康; 呂明偉; 楊莉川; 林全信
• 作者服務機構： 國立中山大學生物系; 國立中山大學海洋資源系
• 中文摘要： 本研究為避免生物本身的磷調節作用干擾此酵素的產生量及胞內磷酸鹽的蓄積量，我們將聚磷酸酵素的操作子換成乳糖的操作子而得到pLl質體。以IPTG誘導含pLl質體的大腸桿菌，結果使得菌體內總磷酸含量大增，然而卻降低此菌的葡萄糖攝取力。由此結果可知，聚磷酸鹽並無法直接當作糖代謝酵素的能量供應者。
• 英文摘要： This paper examines the effects of phosphate pool and expression of polyphosphate kinase on glucoseuptake by expressing the polyphosphate kinase under the control of lac promoter. The E. coli transformant ofpL1, containing an IPTG controllable element for polyphosphate kinase expression, showed that the totalintracellular phosphate significantly increased. However, the rate of glucose uptake by the resting plasmid-bearing cells with IPTG induction significantly decreased. These findings suggest that the polyphosphate cannot directly function as an energy source in E. coli or at least not as a good energy supplier.
• 中文關鍵字： polyphosphate; glucose uptake; E. coli.
• 英文關鍵字： --